Xfree™ Monkeypox Direct PCR Assay
Multi-Platform Availability
The BioGX Xfree™ Monkeypox PCR Assay is offered in the lyophilized “Just Add Sample” format within single PCR tubes where no other reagent is required. The test is available for the BioGX pixl.16 real-time PCR instrument and is also compatible with other real-time PCR instruments. Nucleic acid extraction is not needed, and the lyophilized PCR reagents are simply rehydrated and patient sample is directly added. The fast and simple workflow is designed to help laboratories to increase their testing capacity without adding expert molecular staff or new automation.
Multiplex Configuration
Turnaround Times
Sample Types (validated by laboratory)
Lesion swab samples collected in:
Positive Controls available from BioGX and Microbix
BioGX Lyophilized Positive Controls:
Microbix Positive Swab Control:
Footnote:
aCenters for Disease Control. Preparation of Viral Transport Media (SOP#: DSR-052-05)
bPhosphate Buffered Saline formulation: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4 , 1.8 mM KH2PO4 , pH 7.2-7.4
The BioGX pixl.16 is a high-performance open system real-time PCR platform, equipped with 4-optical channel direct imaging fluorescence, ultra-low-light CMOS bio-imager sensor and fast Peltier-powered thermal cycler to enable reliable and accurate molecular diagnostic testing. This compact device is the ultimate solution for applications where portability, minimal space and fast turnaround times are required.
Add 30μl of Rehydration Buffer to 1 vial of lyophilized PCR reagents. Mix by gently tapping the tube[s] until the reagents are dissolved.
Add 20μl patient sample directly to rehydrated PCR reagents and close the PCR tube strips.
Pulse-spin the sealed PCR tube strips and load samples to BioGX pixl.16 real-time PCR instrument. Start run.
Add 40µl of molecular grade water to 1 vial of lyophilized PCR reagents.
Transfer 10µl of rehydrated master mix to 4 empty tubes and add 5µl of purified nucleic acid.
Pulse-spin the sealed tubes and load to PCR instrument. Start run.
Snap 1 BD MAX™ Extraction Tube into Snap-1 position and snap 1 BD MAX™ empty conical tube into Snap-3 position of each Unitized Reagent Strip.
Add 40µl of BioGX Rehydration Buffer E to 1 vial of lyophilized PCR reagents.
Transfer 40μL of rehydrated PCR reagents to the bottom of each empty conical tube in Snap-3 position.
BioGX Result Analyzer offers a robust, easy-to-use, flexible web-based software solution for analysis of test results from pixl.16 and many other open system platforms.